Methods and Protocols

Author: Sarah J. Hurst

Publisher: Humana Press

ISBN: 9781617790515

Category: Science

Page: 436

View: 9673

Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials. Biomedical Nanotechnology: Methods and Protocols brings together experts from a wide variety of fields to provide a practical overview of biomedical nanotechnology, from the conception of novel materials in the laboratory to the application of such structures in the clinic. After a brief introductory chapter, the first section consists of protocol chapters which provide hands-on information on the synthesis of a variety of solution-phase and surface-bound nanomaterials and their application in sensing, imaging, and/or therapeutics, while the second section consists of a series of case studies and review chapters that discuss the toxicology of nanomaterials, the regulatory pathways to US Food and Drug Administration (FDA) approval of these materials, their patenting, marketing, and commercialization, and the legal and ethical issues surrounding their use. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and insightful tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Biomedical Nanotechnology: Methods and Protocols surveys this exciting field from the most vital angles in order to provide a comprehensive reference for scientists and researchers of all different backgrounds looking to utilize the numerous versatile applications of nanomaterial technologies.
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Author: Chandra P Sharma

Publisher: Elsevier

ISBN: 0323509231

Category: Medical

Page: 451

View: 6832

Drug Delivery Nanosystems for Biomedical Application reviews some of the most challenging nanosystems with different routes of delivery that are useful for specific drugs, from both efficacy and bioavailability points-of-view. The chapters explore how this area is developing, the present state of the field, and future developments, in particular, inorganic, metallic, polymeric, composite and lipid nanosystems and their possible evolution to clinical applications. The book is a valuable research reference for both researchers and industrial partners who are not only interested in learning about this area, but also want to gain insights on how to move towards translational research. Focuses on applications, including tissue engineering and regenerative technologies, showing how nanosystems are used in practice Explores how nanosystems are used to deliver a variety of drugs, including peptides, hormone growth factors and genes Assesses the safety and nanotoxicity aspects of drug delivery nanosystems
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Author: N.A

Publisher: Elsevier

ISBN: 0128139331

Category: Science

Page: 558

View: 9829

Polymeric Nanomaterials in Nanotherapeutics describes how polymeric nanosensors and nanorobotics are used for biomedical instrumentation, surgery, diagnosis and targeted drug delivery for cancer, pharmacokinetics, monitoring of diabetes and healthcare. Key areas of coverage include drug administration and formulations for targeted delivery and release of active agents (drug molecules) to non-healthy tissues and cells. The book demonstrates how these are applied to dental work, wound healing, cancer, cardiovascular diseases, neurodegenerative disorders, infectious diseases, chronic inflammatory diseases, metabolic diseases, and more. Methods of administration discussed include oral, dental, topical and transdermal, pulmonary and nasal, ocular, vaginal, and brain drug delivery and targeting. Drug delivery topics treated in several subchapters includes materials for active targeting and cases study of polymeric nanomaterials in clinical trials. The toxicity and regulatory status of therapeutic polymeric nanomaterials are also examined. The book gives a broad perspective on the topic for researchers, postgraduate students and professionals in the biomaterials, biotechnology, and biomedical fields. Shows how the properties of polymeric nanomaterials can be used to create more efficient medical treatments/therapies Demonstrates the potential and range of applications of polymeric nanomaterials in disease prevention, diagnosis, drug development, and for improving treatment outcomes Accurately explains how nanotherapeutics can help in solving problems in the field through the latest technologies and formulations
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Methods and Protocols

Author: Reema Zeineldin

Publisher: Humana Press

ISBN: 9781493966448

Category: Medical

Page: 490

View: 9313

This detailed volume presents protocols for advancing the utility of nanotechnology in cancer research toward improving our understanding of cancer biology, prevention, diagnosis, and therapy. There are continuous new discoveries in the field of nanotechnology, thus creating new imaging systems or therapies, and this book focuses on how to employ certain discoveries for studying cancer by presenting principles along with techniques to allow for the transformation of any new discoveries in the field into cancer-studying tools with the hope of bringing in the involvement of biomedical scientists who can enhance the speed of discoveries toward cancer diagnosis and therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and motivating, Cancer Nanotechnology: Methods and Protocols serves as an ideal resource for biomedical scientists interested in the potential of this field as well as for physical scientists and engineers interested in employing nanotechnology in cancer diagnosis and therapy./div
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Strategies and Methods

Author: Christof M. Niemeyer

Publisher: Springer Science & Business Media

ISBN: 1592598137

Category: Medical

Page: 330

View: 8090

Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.
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Methods, Devices, and Applications, Second Edition

Author: Tuan Vo-Dinh

Publisher: CRC Press

ISBN: 1439893799

Category: Science

Page: 740

View: 3250

The second edition of Nanotechnology in Biology and Medicine is intended to serve as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of nanotechnology in life sciences. This technology, which is on the scale of molecules, has enabled the development of devices smaller and more efficient than anything currently available. To understand complex biological nanosystems at the cellular level, we urgently need to develop a next-generation nanotechnology tool kit. It is believed that the new advances in genetic engineering, genomics, proteomics, medicine, and biotechnology will depend on our mastering of nanotechnology in the coming decades. The integration of nanotechnology, material sciences, molecular biology, and medicine opens the possibility of detecting and manipulating atoms and molecules using nanodevices, which have the potential for a wide variety of biological research topics and medical uses at the cellular level. This book presents the most recent scientific and technological advances of nanotechnology for use in biology and medicine. Each chapter provides introductory material with an overview of the topic of interest; a description of methods, protocols, instrumentation, and applications; and a collection of published data with an extensive list of references for further details. The goal of this book is to provide a comprehensive overview of the most recent advances in instrumentation, methods, and applications in areas of nanobiotechnology, integrating interdisciplinary research and development of interest to scientists, engineers, manufacturers, teachers, and students.
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Author: N.A

Publisher: Academic Press

ISBN: 0128179104

Category: Business & Economics

Page: 810

View: 7779

Basic Fundamentals of Drug Delivery covers the fundamental principles, advanced methodologies and technologies employed by pharmaceutical scientists, researchers and pharmaceutical industries to transform a drug candidate or new chemical entity into a final administrable drug delivery system. The book also covers various approaches involved in optimizing the therapeutic performance of a biomolecule while designing its appropriate advanced formulation. Provides up-to-date information on translating the physicochemical properties of drugs into drug delivery systems Explores how drugs are administered via various routes, such as orally, parenterally, transdermally or through inhalation Contains extensive references and further reading for course and self-study
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Methods and Protocols

Author: Paolo Bergese,Kimberly Hamad-Schifferli

Publisher: Humana Press

ISBN: 9781627034616

Category: Science

Page: 293

View: 3451

The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.
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Methods and Protocols

Author: Both in the Department of Pharmaceutical Sciences School of Pharmacy Volkmar Weissig,Volkmar Weissig,Tamer Elbayoumi,Mark Olsen

Publisher: Humana Press

ISBN: 9781493962433

Category: Science

Page: 388

View: 1118

This Methods in Molecular Biology book reviews advances in utilizing the potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Includes materials lists, step-by-step protocols and troubleshooting tips."
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Methods and Protocols

Author: Stephen R. Grobmyer,Brij M. Moudgil

Publisher: Humana Press

ISBN: 9781607616139

Category: Medical

Page: 366

View: 3137

Early detection of cancer at the cellular level, even before anatomic anomalies are visible, is critical to more efficacious and cost effective diagnosis and therapeutic advances. In Cancer Nanotechnology: Methods and Protocols, an international panel of experts provide the most recent, cutting-edge, "how-to" approaches developed and employed by researchers in a variety of disciplines to identify cancer specific biomarkers, construct suitable multifunctional targeted nanostructure platforms, along with enhanced imaging and therapeutic applications. Covering such topics as multifunctional and multimodal nanoparticles, nanoparticle mediated cancer theranostics, molecular targets for cancer nanotechnology, and nanoparticles for non-invasive image-guided cancer therapy, the volume addresses the key challenges of the field today, specifically targeted and localized delivery of the drugs. As a volume in the highly successful Methods in Molecular BiologyTM series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Nanotechnology: Methods and Protocols integrates cancer biology, clinical oncology, molecular cancer imaging, materials science and chemical engineering, biomedical engineering, toxicology, computer science, electrical engineering, chemistry, physics, and mathematics in order to achieve the vital goals of nanotechnology-mediated early cancer detection and more efficacious and less toxic therapies for these devastating diseases.
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Author: Sandra Jean Rosenthal,David W. Wright

Publisher: Springer Science & Business Media

ISBN: 159259901X

Category: Science

Page: 228

View: 5081

This volume provides a detailed, hands-on perspective of nanobiotechnology and addresses important, innovative methods in the areas of novel materials synthesis, dynamic cellular imaging and biological assays. Many of the protocols highlight diverse strategies to synthesize and functionalize nanoparticles for biological applications. Other chaptersfocus on the use of biological componrnts (peptides, antibodies and DNA) to synthesize and organize nanoparticles to be used as building blocks in larger assemblies.
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Methods and Protocols

Author: Melba Navarro,Josep A. Planell

Publisher: Humana Press

ISBN: 9781617793875

Category: Science

Page: 319

View: 6168

Nanotechnology plays a key leading role in developing tools able to identify, measure, and study cellular events at the nanometric level as well as in contributing to the disclosure of unknown biological interactions and mechanisms, which opens the door for advances including nanodevices for diagnostic and therapy, drug delivery systems, and regenerative medicine. In Nanotechnology in Regenerative Medicine: Methods and Protocols, expert researchers in the field provide an overview of a very wide range of currently used technologies and methods that involve nanotechnology principles applicable to tissue regeneration. Being that the application of nanotechnology to regenerative medicine is a very broad field, this book focuses its interests on particular areas such as its use as a means to produce efficient platforms and structures for tissue engineering, delivery systems and biosensors, as well as the use of some techniques to study materials surfaces and the interactions between cells, biomolecules, and surfaces at the nanoscale. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their related topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Nanotechnology in Regenerative Medicine: Methods and Protocols provides established scientists, junior researchers, and students involved in the bioengineering, biotechnology, and biomedical fields with a sound foundation in a variety of vital nanotechnology approaches in regenerative medicine.
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Methods and Protocols

Author: Mikhail Soloviev

Publisher: Humana Press

ISBN: 9781493962471

Category: Medical

Page: 576

View: 541

This Methods in Molecular Biology book covers all stages of nanoparticle manufacturing, modification, analysis and application. Includes materials lists, step-by-step, readily reproducible laboratory protocols, troubleshooting tips and avoiding pitfalls."
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Volume II: Micro/Nano Technologies for Genomics and Proteomics

Author: Mihrimah Ozkan,Michael Heller

Publisher: Springer Science & Business Media

ISBN: 0387258434

Category: Technology & Engineering

Page: 540

View: 9636

Volume 2 of the multi-volume reference, BioMEMS and Biomedical Nanotechnology, includes contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices with applications to Genomics and Proteomics. Topics include gene expression profiling utilizing microarray technology; imaging and sensing for gene detection and use in DNA analysis; and coverage of advanced microfluidic devices and the Human Genome Project. This volume is very well illustrated with many of the figures in color.
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Author: Nicole Mattern,Stefan Neuhaus

Publisher: Springer-Verlag

ISBN: 3476046508

Category: Literary Criticism

Page: 312

View: 9028

"Buddenbrooks. Verfall einer Familie" (1901) legte den Grundstein für Thomas Manns außergewöhnliche Karriere als Schriftsteller und Repräsentant der deutschen Literatur und Kultur weltweit. Der Roman zählt nicht nur zu den nobelpreisgekrönten und meistgelesenen, sondern auch zu den meistinterpretierten Texten der deutschsprachigen Literatur. Das Handbuch bündelt die umfassende Forschung über den Roman und präsentiert neben der Entstehungs- und Rezeptionsgeschichte auch die zentralen Themen und Strukturen. Eingegangen wird nicht nur auf Familie, Ökonomie und Religion, sondern auch auf scheinbare Randthemen wie Essen und Trinken oder Elemente des Phantastischen. Zusätzlich eröffnet das Handbuch interpretatorische und literaturtheoretische Zugänge zum Text, von der Sozialgeschichte über Erinnerungs- und Gedächtnistheorien oder Weiblichkeits- und Männlichkeitskonstruktionen bis zur Wissenspoetologie.
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Methods and Protocols

Author: C. Mark Smales,David Cameron James

Publisher: Springer Science & Business Media

ISBN: 1592599222

Category: Medical

Page: 482

View: 9485

This volume contains contributions from leaders in the field of therapeutic protein expression, purification, characterization, formulation and viral inactivation who cover all aspects of protein drug production downstream of the discovery stage. Protocols for the production of therapeutic proteins using a variety of sources are covered. including bacterial and yeast expression systems, insect and mammalian cells. Therapeutic Proteins: Methods and Protocols will prove an invaluable resourse to all those working in the field of therapeutic protein production.
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Biomedical Methods and Applications

Author: Pier Carlo Braga,Davide Ricci

Publisher: Springer Science & Business Media

ISBN: 1592596479

Category: Medical

Page: 394

View: 5097

Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.
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Methods and Protocols

Author: Ehud Gazit,Ruth Nussinov

Publisher: Springer Science & Business Media

ISBN: 9781934115350

Category: Science

Page: 267

View: 3362

As one of the fastest growing fields of research in the 21st century, nanotechnology is sure to have an enormous impact on many aspects of our lives. Nanostructure Design: Methods and Protocols serves as a major reference for theoretical and experimental considerations in the design of biological and bio-inspired building blocks, the physical characterization of the formed structures, and the development of their technical applications. The chapters contributed by leading experts are divided into two sections, the first of which covers experimental aspects of nanostructure design and the second delves into computational methods. As a volume of the highly successful Methods in Molecular BiologyTM series, this collection pulls together cutting-edge protocols, written in a step-by-step, readily reproducible format certain to guide researchers to the desired results. Comprehensive and essential, Nanostructure Design: Methods and Protocols uses biological principles and vehicles on design to aid scientists in the great challenges still ahead.
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Author: Angel Carracedo

Publisher: Springer Science & Business Media

ISBN: 1592598676

Category: Law

Page: 280

View: 6330

In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.
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