Chromatography, Bioconjugation, and Protein Stability
Author: David Pfister,Lucrèce Nicoud,Massimo Morbidelli
Publisher: Cambridge University Press
Provides a coherent and critical view on the potential benefits of various continuous processes in the biopharmaceutical industry.
From Diagnosis to Therapy
Author: Jennifer E. Van Eyk,Michael J. Dunn
Publisher: John Wiley & Sons
Unparalleled in its scope and depth, this book brings together proteomic approaches in diagnosis and treatment from all clinical fields, including clinical toxicology. The result is a new discipline in molecular medicine that will revolutionize the treatment and prevention of cancer, stroke and other severe diseases. Following an overview of clinical proteomics, the authors look at the technologies available, before moving on to cancer, cardiopulmonary disease, diabetes and stroke. A whole section is devoted to toxicity and the work is rounded off with a discussion of the future of clinical proteomics.
Author: Y. H. Hui,Frank Sherkat
Publisher: CRC Press
Category: Technology & Engineering
Advances in food science, technology, and engineering are occurring at such a rapid rate that obtaining current, detailed information is challenging at best. While almost everyone engaged in these disciplines has accumulated a vast variety of data over time, an organized, comprehensive resource containing this data would be invaluable to have. The Handbook of Food Science, Technology, and Engineering, 4 Volume Set is that resource, presenting the latest information available in a thorough, straightforward format.
Author: Danilo Corradini,Elena Eksteen (Katz),Roy Eksteen,Peter Schoenmakers,Neil Miller
Publisher: CRC Press
Delineating its usage in separation, purification and detection processes across a variety of disciplines, from industry to applied research, this work discusses the principles, techniques and instrumentation involving HPLC within a detailed framework. Over 100 tables present previously scattered experimental data.
Author: Hugh M. Cartwright
Publisher: Oxford University Press on Demand
' This area is an intellectually active one that has inspired many research students, not only in chemistry but also engineering, biology and computer science. This book certainly is a useful and valuable reference, written exactly at the level that a computationally literate graduate student can take home, read and find out what is what before deciding whether to delve deeper' Chemistry and IndustryThis is a fundamental reference work for any scientist contemplating using AI for data analysis.
Author: Herman F. Mark
Category: Technology & Engineering
This completely new Third Edition of the Mark Encyclopedia of Polymer Science and Technology brings the state-of-the-art to the 21st century, with coverage of nanotechnology, new imaging and analytical techniques, new methods of controlled polymer architecture, biomimetics, and more. Whereas earlier editions published one volume at a time, the third edition is being published in 3 Parts of 4 volumes each. Each of these 4-volume Parts is an A-Z selection of the latest in polymer science and technology as published in the updated online edition of the Mark Encyclopedia of Polymer Science and Technology (available at www.mrw.interscience.wiley.com/epst). Order the 12 volume set (ISBN 0471275077) now for the best value and receive each of the 4 volume Parts as they publish. The complete list of titles to appear in Part 1 of this new third print edition can be viewed at www.mrw.interscience.wiley.com/epst and clicking on "What's New". Check this website often as new articles are added periodically.
Author: James P. Landers
The Handbook of Capillary Electrophoresis is a valuable collection of in-depth discussions of specific areas of capillary electrophoresis (CE) that are key to the practical use of CE for sample analysis. The handbook is divided into five parts. Part I focuses on various modes of CE that have been developed and applied to analytical/separation problems. Part II covers detection in CE, with one section reviewing all detector systems that have been successfully interfaced with CE and another that focuses on CE-mass spectrometry. Part III explores how CE can be applied to the analysis of a specific class of molecules/analytes or to solving separation problems. CE analysis of organic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments; the components of single cells; and the utility of capillary coatings in CE are all covered in this section. A key element of this handbook is its discussion of the potential role of CE for solving clinical problems and the potential for CE and CE-MS to advance analysis of drug metabolism. The handbook also presents practical and theoretical discussions on various aspects of CE, including sample matrix and its effects on separation, the need for controlling capillary temperature, and the present and future implications of EOF control. In-depth discussions of this nature comprise Parts IV and V. The Handbook of Capillary Electrophoresis will be an outstanding reference for analytical chemists, biochemists, clinical chemists, cell biologists, and pharmaceutical researchers.
Author: Herman Francis Mark
The compact, affordable reference, revised and updated The Encyclopedia of Polymer Science and Technology, Concise Third Edition provides the key information from the complete, twelve-volume Mark's Encyclopedia in an affordable, condensed format. Completely revised and updated, this user-friendly desk reference offers quick access to all areas of polymer science, including important advances in nanotechnology, imaging and analytical techniques, controlled polymer architecture, biomimetics, and more, all in one volume. Like the twelve-volume full edition, the Encyclopedia of Polymer Science and Technology, Concise Third Edition provides both SI and common units, carefully selected key references for each article, and hundreds of tables, charts, figures, and graphs.
Principles, High Resolution Methods, and Applications
Author: Jan-Christer Janson
Publisher: John Wiley & Sons
The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography
Author: M. Kastner
Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.
Author: R. E. Ardrey
Publisher: John Wiley & Sons
This series of books provides coverage of all of the majoranalytical techniques and their application in the most importantareas of physical, life and materials science. Each text ispresented in an open learning/distance learning style, in which thelearning objectives are clearly identified. The reader'sunderstanding of the material is constantly evaluated by the use ofself-assessment and discussion questions. The combination of gas chromatography with mass spectrometry(GC-MS) has been a routine analytical tool for many years, whilethe related hybrid technique of liquid chromatography-massspectrometry (LC-MS) is a rather more recent development. However,with the advent of new interfacing technology, the benefits of thispowerful hybrid analytical method are now currently being realised,as witnessed by the exponential growth in applications of thistechnique in both the chemical and life sciences. The topics covered in this text include the following: * The basic principles of liquid chromatography and massspectrometry * The limitations of the component techniques when used inisolation, and how a combination of the two allows these to beovercome * Descriptions of the various approaches, including thermospray,electrospray and atmospheric pressure chemical ionization, whichare used to interface the two techniques, along with the advantagesand disadvantages of each system * Illustrative examples of the applications of LC-MS, including themolecular weight and structure determinations of both biopolymersand low-molecular-compounds, along with the development ofquantitative methods Key features include: * The provision of many discussion and self-assessment questionsthroughout the text, thus providing opportunities for testing thereader's understanding of the subject matter * Presented in the AnTS (open learning/distance learning) style, itis ideal for use as both a self-study guide and as the basis of ataught course * A comprehensive bibliography, including books, papers/articles injournals and on websites, along with specific references for theindividual chapters, to guide the reader to more specialised textsand original sources Written with a student audience in mind, this text will provide aninvaluable introduction to this increasingly important analyticaltandem technique.
First multi-year cumulation covers six years: 1965-70.
Author: H. Engelhardt
Die Hochdruck-Flüssigkeits-Chromatographie entwickelt sich seit etwa 1969 mit großer Geschwindigkeit zu einer Standard-Trennmethode. Geprägt wurde die Entwicklung dadurch, daß ihre "Pioniere" fast ausschließlich Experten der Gas-Chromatographie gewesen sind. Sie glaubten, die Prin zipien und Methoden der alten Methode auch im Bereich der schnellen Flüssigkeits-Chromatographie voll anwenden zu können. Es hat eine Weile gedauert, bis endgültig klar wurde, daß dies nicht so ist. Zwar ist es für die Theorie der Chromatographie gleich, ob der Eluent ein Gas oder eine Flüssigkeit ist, die quantitativen Unterschiede der Parameter (wie Viskosität, Diffusionskoeffizient) ändern das Bild aber wesent lich. Heute scheint allgemein akzeptiert zu werden, daß das Apparative der Hochdruck-Flüssigkeits-Chromatographie sogar einfacher als bei der Gas-Chromatographie ist. Weiterhin scheint man einig, daß optimale Analysen mit stationären Phasen mit Siebfraktion um 5 oder 10 ~m durch zuführen sind. Da die Entwicklung kommerzieller Geräte etwa zwei Jahre in An spruch nimmt, ist es selbstverständlich, daß heute noch eine Lücke zwischen dem neuesten Stand der Technik und den kommerziell angebote nen Geräten klafft. Es ist sehr viel getan, wenn es mit diesem Buch gelingt, dem Praktiker jene Fragen nahezubringen, die er sich stellen muß, bevor er ein hochdruck-flüssigkeits-chromatographisches Gerät anschafft, und wenn ihm das Auffinden des geeigneten Systems (statio näre Phase und Eluent) für sein spezifisches Trennproblem erleichtert wird. Das gelingt auch ohne detaillierte Diskussion der Theorie der Chromatographie.